TABLE 3.
VP7 primers and type-specific probes used for amplification and genotyping
| Primer or probe | Sequence | Positions | Reference or source |
|---|---|---|---|
| Primers for RT-PCR | |||
| Beg9 | GGCTTTAAAAGAGAGAATTTCCGTCTGG | 1-28 | 27 |
| End9 | GGTCACATCATACAATTCTAATCTAAG | 1036-1062 | 27 |
| sBeg9 | GGCTTTAAAAGAGAGAATTTC | 1-21 | 28 |
| RVG9 | GGTCACATCATACAATTCTAATCTAAG | 1044-1062 | 27 |
| LID1 | ATGTATGGTATTGAATATACCA | 48-70 | 11 |
| 9Con1 | TAGCTCCTTTTAATGTATGG | 37-56 | 15 |
| G922 | GTRTARAAIACTTGCCACCA | 922-941 | 11 |
| G-type-specific probes | |||
| G1-2 | ATATAGTTGAGTAGAATRATTG | 92-114 | 11 |
| G1-3 | ATRTAGTTGAGTAAAATAACTG | 92-114 | 11 |
| G1-4 | CCATTATTCGRGTCACTGATT | 119-139 | 11 |
| G2-2 | GTCCATYGTATTAGTTATAGTTT | 119-141 | 11 |
| G2-3 | CTYACAAATGGTGAYATCAGA | 172-194 | 11 |
| G2-5 | AATTCGTATATRCAGCGTCTA | 136-156 | 11 |
| G3-13 | GAGCAACTGAAAASGADGCAAT | 418-439 | 36 |
| G3-15 | ATGGTGACARNATAACTAYAAT | 166-187 | This paper |
| G3-16 | TCATTTATTYCWGTNGCAGCTT | 311-332 | This paper |
| G4-1 | TATCAARTAAAAATAGAACTGTG | 59-90 | 11 |
| G4-2 | TAACTCACAAGAACGAACGATATCAA | 85-111 | 11 |
| G4-3 | GTTTTYARAATRTAACTCACAAG | 100-122 | 11 |
| G5-5 | GAAAATGAYGCAATATCTGCATA | 409-431 | 36 |
| G5-6 | AGACATRTCYAACTGTAAATTT | 489-507 | 36 |
| G5-7 | AGCCACTGTYTCAAAYGAATTT | 684-704 | 36 |
| G6-6 | TTTTACAGTTGCGTATNGTACA | 778-799 | 36 |
| G6-8 | ATTCAACICTAGAATTRGATA | 482-502 | This paper |
| G6-9 | GTTATCCAAGTAGGCGGCGCGAAC | 826-849 | This paper |
| G8-1 | TAATTCAATAATACWAGCAATAT | 88-110 | 36 |
| G8-5 | GTTTCWGCTTCYACTGGRTAATA | 298-320 | 36 |
| G9-3 | TTCTTGCTGTGATGAATTTG | 251-270 | 11 |
| G9-4 | GTATCTAGCTGTGATGAGTTTG | 251-272 | 11 |
| G9-5 | ATCTCCAATTTGAGTTGATGC | 313-333 | 11 |
| G10-3 | TTWGTAGCRTTCACATATGAC | 243-263 | 36 |
| G10-5 | AARGTAGSTATATCATCRTATT | 407-428 | 36 |
| G10-6 | ATTTGTRGTCTGACATCCTAT | 661-681 | 36 |
| G12-2 | TCGGGRTCAGTTATTTCAGTCGT | 316-338 | 36 |
| G12-6 | TTCAAATGTTGYGACGTCGGTTG | 674-696 | 36 |
| G12-7 | CATGTATTCAMTGTAATATT | 760-779 | 36 |