TABLE 4.
VP4 primers and type-specific probes used for amplification and genotyping
| Primer or probe | Sequence | Positions | Reference or source |
|---|---|---|---|
| Primers for RT-PCR | |||
| Con3 | TGGCTTCGCTCATTTATAGACA | 11-32 | 24 |
| Con2 | ATTTCGGACCATTTATAACC | 868-887 | 24 |
| F4 | TATAAAATGGCTTCIYTVAT | 4-23 | 36 |
| C8 | ATTGGIGTYTGATAITCRTC | 1446-1467 | 36 |
| P-type-specific probes | |||
| P4-5 | ACYCTGACTAYTACCTTTAAAC | 439-460 | 36 |
| P4-6 | AGTYCGTCTATTAGARAA | 464-481 | 36 |
| P4-7 | TTAGCATYCCTACRAGTCTATT | 494-515 | 36 |
| P6-1 | GTRCCCTCTAATACAACYTGTTGAT | 272-297 | 36 |
| P6-2 | GTTTCTCCAAAYAAYGTGTATTG | 362-384 | 36 |
| P6-4 | AGTGYGCTTATGTTGAAATTCGG | 459-481 | 36 |
| P8-2 | TAACGTGCGGTTCAAYAGCAAC | 324-346 | 36 |
| P8-4 | AATCRTTICTYACATTAAATTG | 388-409 | 36 |
| P8-5 | AAKTCAYTTTGACTACTGCTT | 441-461 | 36 |
| P9-1 | GTACCTTCAGCAACTYTYCC | 278-297 | 36 |
| P9-2 | TTGGACATTTYGCCCATCTAA | 371-391 | 36 |
| P9-4 | AATTCRGCGTCRCTTGAAAC | 605-624 | 36 |
| P14-1 | GCYACYCTWCCTATTTGAGT | 269-288 | 36 |
| P14-3 | AATTTCCAAAGRGTRCTTGA | 410-429 | 36 |
| P14-4 | ACTCTYCCTTCTCTYTTYATCCA | 509-531 | 36 |