TABLE 5.
G and P genotypes of stool rotavirus samples determined by typing PCR in each country of origin and PCR-ELISAs in this study
| Country of origin of stool samples | Yr(s) of sample collection | No. of samples analyzed | Genotype determined by:
|
|||
|---|---|---|---|---|---|---|
| Typing PCR
|
PCR-ELISA
|
|||||
| G | P | G | P | |||
| Brazil | 1996-2004 | 1 | G1 | P[4] | G1 | P[8] |
| 1 | G1 | P[6]+[8] | G1 | P[8] | ||
| 61 | G1 | P[8] | G1 | P[8] | ||
| 8 | G1 | P? | G1 | P[8] | ||
| 1 | G1 | P? | G9 | P[8] | ||
| 11 | G1 | P[8] | G9 | P[8] | ||
| 2 | G1+G9 | P[8] | G1+G9 | P[8] | ||
| 1 | G2 | P[4] | G2 | P[4] | ||
| 1 | G? | P[4] | G2 | P[4] | ||
| 1 | G? | P? | G3 | P[8] | ||
| 1 | G? | P? | G3 | P? | ||
| 1 | G4 | P[8] | G4 | P[8] | ||
| 1 | G5 | P[6] | G5 | P[8] | ||
| 10 | G5 | P[8] | G5 | P[8] | ||
| 1 | G6+G9 | P[8] | G9 | P[8] | ||
| 1 | G8 | P[4] | G8 | P[4] | ||
| 2 | G9 | P[8] | G9 | P[8] | ||
| 1 | G10 | P[9] | G10 | P[9] | ||
| Subtotal | 106 | |||||
| 40 | G1 | P[8] | G1 | NT | ||
| 6 | G1+G2 | P[8] | G1 | NT | ||
| 3 | G1+G4 | P[8] | G1 | NT | ||
| 55 | G4+G9b | P[8] | G9 | NT | ||
| 12 | G4 | P[8] | G4 | NT | ||
| 34 | G9 | P[8] | G9 | NT | ||
| Subtotal | 150 | |||||
| Ghana | 2003-2004 | 1 | G1 | P[8] | G9 | P[8] |
| 1 | G2 | P[4] | G8 | P[4] | ||
| 4 | G2 | P[6] | G2 | P[6] | ||
| 2 | G2 | P[6]+P[10] | G2 | P[6] | ||
| 1 | G2 | P[8] | G9 | P[8] | ||
| 2 | G3 | P[4] | G8 | P[4] | ||
| 1 | G3 | P[6] | G3 | P[6] | ||
| 1 | G3 | P? | G9 | P[8] | ||
| 1 | G9 | P[6]+[10] | G9 | P[8] | ||
| 11 | G9 | P[8] | G9 | P[8] | ||
| 2 | G? | P[8] | G9 | P[8] | ||
| 1 | G? | P[8] | G2+G9 | P[8] | ||
| Subtotal | 28 | |||||
| 1 | NT | NT | G1 | P[6] | ||
| 6 | NT | NT | G1 | P[8] | ||
| 2 | NT | NT | G1 | P? | ||
| 1 | NT | NT | G1+G9 | P[8] | ||
| 1 | NT | NT | G3 | P? | ||
| 2 | NT | NT | G9 | P[8] | ||
| 4 | NT | NT | G10 | P[6] | ||
| Subtotal | 17 | |||||
| Japan | 2003 | 9 | NT | NT | G1 | P[8] |
| 1 | NT | NT | G3 | P[4] | ||
| 22 | NT | NT | G3 | P[8] | ||
| 25 | NT | NT | G4 | P[8] | ||
| 5 | NT | NT | G9 | P[8] | ||
| 1 | NT | NT | G? | P[8] | ||
| 1 | NT | NT | G? | P? | ||
| Subtotal | 64 | |||||
| United States | 1997-2000 | 30 | G1 | P[8] | G1 | P[8] |
| 1 | G1 | P[8] | G1 | P[6] | ||
| Subtotal | 31 | |||||
| Total | 396 | |||||
aDiscrepant G and/or P types are in boldface type. ?, nontypeable by the indicated assay; NT, not tested.
b
These samples were reanalyzed in this study using three different primer pools and correctly typed as being G9 by use of the C pool as indicated in the text.