TABLE 2.
Oligonucleotide primers used
| Target | Primer name | Primer sequence (5′→ 3′) | Reference or source |
|---|---|---|---|
| 16S-23S rRNA intergenic region | QHVE1 | TTCAGATGATGATCCCAA | 23 |
| QHVE4 | AACATGTCTGAATATATC | ||
| 16S rRNA gene | Rick16SF1 | GTATGCTTAACACATGCAAGTCGAAC | 39 |
| Rick16SR4 | TCCGCGATTACTAGCGATTCC | ||
| ftsZ | FtsZ F (Bfp1) | ATTAATCTGCAYCGGCCAGA | 44 |
| FtsZ R (BatfsZR) | GCTGGTATTTCCAAYTGATCT | ||
| rpoB | rpoB F (1400F) | CGCATTGGCTTACTTCGTATG | 41 |
| rpoB R (2200R) | GTAGACTGATTAGAACGCTG | ||
| gltA | BhCS.781p | GGGGACCAGCTCATGGTGG | 37 |
| BhCS.1137n | AATGCAAAAAGAACAGTAAACA | ||
| ribC | RibC-1F | CGGATATCGGTTGTGTTGAA | 45 |
| RibC-1R | CATCAATRTGACCAGAAACCA | ||
| RibC-2F | GCATCAATTGCGTGTTCA | ||
| RibC-2R | CCCATTTCATCACCCAAT | ||
| htrA | CAT-1 | GATTCAATTGGTTTGAA(A/G)GAGGCT | 3 |
| CAT-2 | TCACATCACCAG G(A/G)CGTATTC | ||
| CAT-FN | AAGCTGGTATCAAGGCAG | J. Sumner, personal communication | |
| CAT-RN | CCCATCATCAGAAGGAGC |