TABLE 2.
eap-targeting oligonucleotide primers used in this study
| Name | Oligonucleotide sequence (5′→3′) | Amplicon size (bp) | Source or reference |
|---|---|---|---|
| EAP-CON1 | TAC TAA CGA AGC ATC TGC C | 230 | This study |
| EAP-CON2 | TTA AAT CGA TAT CAC TAA TAC CTC | ||
| EAP-P3wt | GCA GCT AAG CCA TTA GAT AAA TCA TCA AGT TGC TTA CAC C | Variablea | Derived from primer P3 (19)b |
| EAP-P2wt | TTA AAA TTT AAT TTC AAT GTC TAC TTT TTT AAT GTC |
a
Strain-specific amplicon size depending on the number of tandem repeats in the eap gene.
b
The primer sequences were modified by omitting the originally introduced restriction sites.