TABLE 2.
Oligonucleotides used for PCR or DNA sequencing
| Gene | Primer sequencea | Position in Rd genome |
|---|---|---|
| adk | F: GGTGCACCGGGTGCAGGTAA | 376521 |
| R: CCTAAGATTTTATCTAACTC | 375899 | |
| pgi | F: GGTGAAAAAATCAATCGTAC | 1644657 |
| R: ATTGAAAGACCAATAGCTGA | 1645246 | |
| recA | F: ATGGCAACTCAAGAAGAAAA | 622556 |
| R: TTACCAAACATCACGCCTAT | 621940 | |
| infB | F: TGAAAATGAGCTTGAAGAAGCGG | 1361550 |
| R: GATAGTTGCCACAGGGCCACGACC | 1362198 | |
| 16S rRNA | F1: CCAGCAGCCGCGGTAATACG | 624342 |
| R1: ATCGGYTACCTTGTTACGACTTC | 625335 | |
| F2: GCCCGCACAAGCGGTGGAGCATGTG | 624751 | |
| R2: CTCGTAAGGGCCATGATGACTTGACG | 625039 | |
| iga | F: GTTCCACCACCTGCGCCTGCTAC | 1050386 |
| R: GTTATATTGCCCCTCGTTATTCA | 1049130 | |
| licAb | F: GTAGGATTTGTTAAAACTTGCTACAAGCC | 1608693 |
| R: GGCAATTCCTCTAACAGTTTAAATGCTGCG | 1609579 | |
| lic2Ab | F: ATATTACATAATATAGAGGAATCTAG | 571382 |
| R: CTACATAAAACGAACAATTTCTTTACC | 570690 | |
| lgtCb | F: CGGACTGTCAGTCAGACAATG | 289338 |
| R: CTCAAAATGATCATACCAAGATG | 288499 |
a
All oligonucleotides are based on the DNA sequences from H. influenzae strain Rd. F, forward, R, reverse.
b
The forward primers begin downstream of tetranucleotide repeats.