TABLE 1.
Gene | Primer designation | Primer sequence (5′ to 3′)a | Product size (bp) |
---|---|---|---|
For cloning into S. aureus RN4220 | |||
erm(A) | erm(A)BH | + CAGTGGATCCTCTTATCAAG | 1,107 |
erm(A)XB | − CTTGTGATCTAGAGAACGC | ||
erm(C) | erm(C)BH | + TTAGATGGATCCCTCATATC | 999 |
erm(C) XB | − TGCATCTCTAGACTTACTTATT | ||
For sequencing of the translational attenuator | |||
erm(A) | erm(A)1LP | + TTTTTAGTAAAGACAGTGGC | 481 |
erm(A)2LP | − GTCCTTTTCCTGATCCGA | ||
erm(C) | erm(C)1LP | + TACAAGAAAAAAGAAATTAG | 327 |
erm(C)2LP | − ATCTATATTATGTTTTGAAG |
+, sense primer; −, antisense primer.