TABLE 1.
Primers used in this study
| Gene | Primer designation | Primer sequence (5′ to 3′)a | Product size (bp) |
|---|---|---|---|
| For cloning into S. aureus RN4220 | |||
| erm(A) | erm(A)BH | + CAGTGGATCCTCTTATCAAG | 1,107 |
| erm(A)XB | − CTTGTGATCTAGAGAACGC | ||
| erm(C) | erm(C)BH | + TTAGATGGATCCCTCATATC | 999 |
| erm(C) XB | − TGCATCTCTAGACTTACTTATT | ||
| For sequencing of the translational attenuator | |||
| erm(A) | erm(A)1LP | + TTTTTAGTAAAGACAGTGGC | 481 |
| erm(A)2LP | − GTCCTTTTCCTGATCCGA | ||
| erm(C) | erm(C)1LP | + TACAAGAAAAAAGAAATTAG | 327 |
| erm(C)2LP | − ATCTATATTATGTTTTGAAG |
a
+, sense primer; −, antisense primer.