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Sensitivity of duplex real-time PCR when coamplification was performed
| Coamplification of DNA | Detection (CT value) of DNA from:
|
|||
|---|---|---|---|---|
|
C. pneumoniae
|
M. pneumoniae
|
|||
| 10 copies | 104 copies | 10 copies | 104 copies | |
| None | 33.9 ± 0.7 | 24.3 ± 0.08 | ||
| M. pneumoniae (10 copies) | 33.9 ± 0.7 | 24.5 ± 0.3 | ||
| M. pneumoniae (104 copies) | 32.2 ± 0.5 | 23.7 ± 0.02 | ||
| None | 30.2 ± 0.3 | 20.8 ± 0.3 | ||
| C. pneumoniae (10 copies) | 30.7 ± 0.2 | 20.5 ± 0.5 | ||
| C. pneumoniae (104 copies) | 29.9 ± 0.2 | 20.8 ± 0.05 | ||
