TABLE 1.

Clinical and demographic features of a cohort of cats with lesions likely to be attributable to Mycobacterium sp. strain Tarwin^a

Case no., name of cat, and yr of diagnosis	Breed, age (yrs)	Gender	Clinical sign(s)	Pathology and biopsy findings (specimen available for DNA extraction)	Lifestyle	Sequence data obtained
1, Nina, 1999 (case 2 in reference 17)	DSH, 2	FN	Caudal right hock, then lateral digit on left forelimb	Numerous AFB, branching morphology, no necrosis, routine culture negative (lyophilized tissue)	Indoor/outdoor, occasional hunter	16S rRNA, ITS1, hsp65
2, Sasha, 2002 (case 1 in McCowan et al., submitted)	DLH, 6	FN	5-mo history of raised corneal mass	Numerous AFB within macrophages, scanty growth in BACTEC 12B at 31°C, no growth on subculture (BACTEC broth culture)	Indoor/outdoor	16S rRNA, ITS1
3, Susie, 2003 (case 2 in McCowan et al., submitted)	Burmese cross, 17	FN	Cloudy eye that progressed to a thickened, red corneal mass	Numerous AFB, grew in BACTEC 12B at 31°C, no growth on subculture (fresh corneal tissue)	Predominantly indoor	16S rRNA, ITS1
4, Centrals, 2005	DLH, 3	MN	Chin nodule and multiple nodules on head and feet	Many AFB in biopsy tissue (frozen tissue sample)	History of fighting	16S rRNA, ITS1
5, Simba, 2005	DSH, 4.5	MN	Mouth and digit nodules	NA (digit nodule tissue)	NA	16S rRNA, ITS1, hsp65, rpoB, sodA
6, Jagula, 2005	Abyssinian, 8	FN	Firm, rapidly growing mass over frontal sinus; bone under the lesion was roughened	NA (MGIT broth culture)	NA	ITS1
7, Jazzy, 2005	DLH, 4	MN	Eyelid nodule, 1-cm diam	FNA showed macrophages with negatively stained bacilli (eyelid tissue)	Indoor/outdoor, history of cat fight with scratches	ITS1, hsp65
8, Runtie, 2005	DSH, 3	MN	First lesion on right front digit (8-mm nodule), second lesion on left forearm (20-mm nodule)	Biopsy showed multibacillary, lepromatous tissue (paraffin sections, forearm tissue)	Indoor/outdoor, hunter	16S rRNA, ITS1, hsp65
9, Steel, 2005	DLH, 5	MN	Multiple nodules, head and feet	Biopsy showed multibacillary, lepromatous tissue (paraffin sections, head tissue)	Indoor/outdoor, hunter	16S rRNA, ITS1, hsp65, rpoB
10, Jack, 2004	DSH, 3	MN	Injury between digits on left rear foot turned into an ulcerated mass, removed surgically; 4 mo later developed a grape-sized enlargement of regional popliteal lymph node	Biopsy of interdigital lesion showed epithelial macrophages, lymphocytes, plasma cells, neutrophils plus AFB; FNA showed popliteal lymph node lymphocytes, plasma cells, and macrophages containing NSB (paraffin sections)	Indoor/outdoor, hunter	16S rRNA, ITS1, hsp65, rpoB
11, Zoe, 2003	DSH, 10	FN	1-mo history of stridor/stertor and a soft-tissue mass above the upper incisors; granulomas in caudal nasal cavity	Masses of AFB in FNA of mass and nasal wash; no growth on routine culture (lyophilized tissue)		16S rRNA, ITS1, hsp65, rpoB
12, Old Tom, 2006	DSH, 6	MN	Lumps on upper and lower eyelids	Large no. of AFB seen in tissue sections (paraffin sections)		16S rRNA, ITS1, hsp65, rpoB
13, Pepper, 2006	DSH, 13	MN	Swelling (4 mm) lateral to nose with ipsilateral epiphora	Biopsy showed abundant epithelioid macrophages, giant cells, packed with AFB (paraffin sections)	Mainly outdoor, hunter	ITS1
14, Fang, 2004	DSH, 2	FN	Lump on left forelimb and left carpal area (2-cm diam), dermal plaque with ulcerated surface	FNA of lesion showed macrophages containing NSB (NA)	Indoor/outdoor, known hunter with access to rats and mice	NA
15, Hoover, 2005	DMH, 13.5	MN	Mass on mandible just under chin (1-cm diam), painful, domed, hairless, smooth	FNA of lesion showed neutrophils and macrophages containing NSB (NA)	Mainly outdoor	NA

^aGenetic loci for which nucleotide sequence information were obtained also are shown. Abbreviations: NA, not available; DSH, domestic short haired; DLH, domestic long haired; DMH, domestic medium haired; MN, male neutered; FN, female neutered; NSB, negatively stained bacilli; FNA, fine-needle aspirate.