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Sensitivity of detection of adenovirus serotype 4 genomic material by PCR using primer pairs PP769 and PP943 as evaluated by ESI-MS-based analysis. Adenovirus serotype 4 genomic material was present at 10, 100, or 1,000 PFU per PCR. (A) ESI-MS detection following amplification by PP943. (B) ESI-MS detection following amplification by PP769. The two peaks in each spectrum correspond to sense and antisense strands of the PCR amplicons, which separate under the conditions of ESI.
