TABLE 2.
Comparison of PCR/ESI-MS detections with those of a standard BCE-2 PCR assay
| Identification of previously characterized sample | Identification by:
|
Base count for T5000 detection with primer pair:
|
||
|---|---|---|---|---|
| T5000 | Standard BCE-2 PCR | PP943 | PP769 | |
| Cultured isolates | ||||
| Ad C1 | Ad C | C | A20 G33 C39 T20 | Does not prime |
| Ad C5 | Ad C5 | C | A20 G33 C39 T20 | A33 G27 C35 T26 |
| Ad C6 | Ad C6 | C | A20 G33 C39 T20 | A31 G29 C34 T27 |
| Ad B3 | Ad B3 | C | A23 G31 C37 T21 | A33 G24 C37 T27 |
| Ad B7 | Ad B7 | B | A23 G32 C36 T21 | A33 G24 C36 T28 |
| Ad B21 | Ad B21 | B | A21 G33 C37 T21 | A31 G28 C35 T27 |
| Ad B14 | Ad B14/B34 | B | A21 G33 C37 T21 | A31 G30 C34 T26 |
| Ad B16 | Ad B16 | B | A23 G32 C36 T21 | A31 G30 C34 T26 |
| Ad F40 | Ad F40 | C | A21 G33 C39 T19 | Does not prime |
| Clinical specimens | ||||
| Ad B7 | Ad B7 | B | A23 G32 C36 T21 | A32 G24 C37 T28 |
| Ad B14 | Ad B14/B34 | B | A21 G33 C37 T21 | A31 G30 C34 T26 |
| Ad E4a | Ad E4a | E4a | A20 G33 C39 T20 | A30 G31 C38 T22 |
| Ad E4p | Ad E4p | E4p | A21 G33 C37 T21 | A27 G33 C39 T22 |