FIG. 1.

Aspergillus virus 178, virus 1816, and virus 341. The sizes of the longest visible dsRNA elements associated with virus 178 (∼6 kb), virus 1816 (∼3.7 kb), and virus 341 (∼3.6 kb) were estimated by their migration distance relative to a DNA ladder during gel electrophoresis. Sequencing of cDNA from these dsRNA elements resulted in contigs that span part (virus 187), most (virus 1816), or all (virus 341) of their lengths. Two separate contigs were obtained for virus 178, and their predicted positions are indicated. Putative domains are shown (coat protein [pfam05518] and RdRP [pfam02123]). The Expect value for the virus 178 coat protein domain is 2e-84 and for the virus 178, 1816, and 341 RdRP domains are 6e-97, 2e-43, and 3e-11, respectively. The positions of oligonucleotides o1 to o6, which were used as controls for siRNA screening, are indicated. Sense oligonucleotides are depicted above the contig, and antisense oligonucleotides are depicted below the contig. Probe templates and the region used for v341 IRT construction are also indicated.