TABLE 1.
Strains used in this study
| Category and straina | Genotype |
|---|---|
| Reference strains | |
| RTMH13.B3* | ΔstcE::argB; veA1 |
| RTMH13.B1* | ΔstcE::argB; aflR(IRT)::trpC veA1 |
| RTMH13.F2 | pyrG89; metG1, ΔstcE::argB; veA1 |
| RTMH13.F3* | pyrG89; metG1 ΔstcE::argB; aflR(IRT)::trpC veA1 |
| Transformants | |
| TEAB65.C1‡ | pyrG89; metG1 ΔstcE::argB; ΔrsdA::pyrG; aflR(IRT)::trpC veA1 |
| TJW64.17, 46 | pyrG89; wA3; pyroA4 ΔstcE::argB; ΔdclA::pyroA, trpC801 veA1 |
| TBRG3.6 | pyrG89; ΔppdB::metG; metG1 ΔstcE::argB; aflR(IRT)::trpC veA1 |
| TTMH158.1 | pyrG89; metG1 ΔstcE::argB; ΔdclB::pyrG; aflR(IRT)::trpC veA1 |
| TTMH160.3 | pyrG89; wA3; gpdA(p)::dclB::pyroA ΔstcE::argB; veA1 |
| Analysis of dclA function in IRT-RNA silencing (Fig. 3) | |
| RTMH192.1 | pyrG89; wA3; ΔstcE::argB; veA1 |
| RTMH192.2, 5, 10 | pyrG89; ΔstcE::argB; aflR(IRT)::trpC ΔdclA::pyroA veA1 |
| RTMH192.3, 6, 8 | pyrG89; ΔstcE::argB; aflR(IRT)::trpC veA1 |
| RTMH192.4 | pyrG89; ΔstcE::argB; ΔdclA::pyroA, veA1 |
| RTMH192.7 | pyrG89; wA3; ΔstcE::argB; aflR(IRT)::trpC, ΔdclA::pyroA veA1 |
| RTMH192.9 | pyrG89; wA3; ΔstcE::argB; aflR(IRT)::trpC, veA1 |
| Analysis of ppdB and rsdA function in IRT-RNA silencing (Fig. 4) | |
| RTMH193.1‡ | pyrG89; ΔppdB::metG; metG1 ΔstcE::argB; ΔrsdA::pyrG; aflR(IRT)::trpC veA1 |
| RTMH193.4 | pyrG89; metG1 ΔstcE::argB; ΔrsdA::pyrG; aflR(IRT)::trpC veA1 |
| RTMH193.10 | pyrG89; ΔppdB::metG; ΔstcE::argB; aflR(IRT)::trpC veA1 |
| Analysis of dclB function in IRT-RNA silencing (Fig. 7) | |
| RTMH215.6, 7 | pyrG89; ΔstcE::argB; ΔdclB::pyrG; veA1 |
| RTMH215.12, 13 | pyrG89; ΔstcE::argB; ΔdclB::pyrG; aflR(IRT)::trpC veA1 |
| RTMH215.4, 17 | pyrG89; gpdA(p)::dclB::pyroA ΔstcE::argB; ΔdclB::pyrG; veA1 |
| RTMH215.20, 29 | pyrG89; gpdA(p)::dclB::pyroA ΔstcE::argB; ΔdclB::pyrG; aflR(IRT)::trpC veA1 |
| Analysis of radial growth and spore production (Table 3) | |
| RDIT9.32† | WT |
| RTMH211.14 | WT |
| RTMH229.24 | WT |
| RTMH218.7, 39 | ΔrrpB::pyrG; ΔrrpC::metG ΔdclB::pyrG; ΔrsdA::pyrG |
| RTMH211.6, 11 | ΔppdB::metG |
| RTMH212.42, 65 | ΔdclA::pyroA |
| Analysis of crossing (Table 4) | |
| RTMH227.29, 47 | yA2; ΔrrpB::pyrG; ΔrrpC::metG ΔdclB::pyrG; ΔrsdA::pyrG |
| RTMH227.67, 68 | wA3; ΔrrpB::pyrG; ΔrrpC::metG ΔdclB::pyrG; ΔrsdA::pyrG |
| RTMH229.25, 37 | yA2 |
| RTMH229.19 | wA3 |
| RTMH233.C | wA3 |
a
Commas are used between the suffixes of strain names to indicate independently isolated transformants or recombinants of the same genotype. The aflR IRT transgene used in this study is the aflR(IRT1300) transgene referenced earlier (30). Strain sources: *, Hammond and Keller (30), †, Tsitsigiannis et al. (69). ‡, Strains that carry an ectopic copy of the rsdA deletion vector; this does not appear to have influenced any of the results (data not shown). Additional strains used in this study, such as the transformation hosts and crossing intermediates, are described in the supplemental material.