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2D gel electrophoresis analysis of eEF2 isoforms after stress treatments. Wild-type (upper row) or sty1 mutant (lower row) cells expressing HA-tagged eEF2 were treated as indicated. Lysates were prepared and proteins separated on 2D gels, blotted, and probed with anti-HA antibodies. The arrows (A, C, I, and J) point to the predominant isoform.
