FIG. 4.
Complementation of the yeast mep1 mep2 mep3 triple mutant with the F. fujikuroi mepA, mepB, and mepC cDNA fragments under the control of the S. cerevisiae GAL1 promoter. All three mep genes fully restored the growth of the mep1 mep2 mep3/mep1 mep2 mep3 mutant on SLADG minimal medium. Transformation of the diploid yeast mep2/mep2 mutant with the F. fujikuroi mepA and mepC genes, but not with the F. fujikuroi mepB gene, restored pseudohyphal growth on the nitrogen limited medium SLADG.