FIG. 1.

Southern analysis of Δcch1 mutants and complements compared to the wild type using a probe internal to CCH1 (A) and a probe specific to hph1 (B). Lanes from left to right: 1-kb ladder, wild type (WT), transformant Δcch1-T10, Δcch1-T11, Δcch1-T12, Δcch1-T13, Δcch1-T14, and complements cch1comp6 and cch1comp5. There is an empty lane between the two complements. Transformant Δcch1-T11 is an ectopic insertion of the replacement construct, including hph. Ten micrograms was loaded per lane. Genomic DNA was digested with BamHI. The CCH1 internal probe hybridizes to a BamHI fragment of 2,891 bp in the wild type. The hph1 probe hybridizes to a BamHI fragment of 3,465 bp in Δcch1 replacement mutants. Numbers on the left indicate size in kb.