TABLE 1.

Primers used in this study

Primer no.	Primer name	Sequence (5′-3′)	Contiga	Coordinatesa
1	Cch1 U5	GATATCCAGTCGCGTCAC	1.69	11544-11527
2	Cch1 U3	ACTTATTCAGGCGTAGCAACCAGGCGTCCAACGAGGTCCAATGTC	1.69	11107-11124
3	Cch1 U5 nested	ATGTTCGAAGCGTGTTTAGTGTGTTT	1.69	11524-11499
4	Cch1 D5	TCAGCATCTTTTACTTTCACCAGCGTTGGAAGAGAAGATAGGTAC	1.69	4504-4487
5	Cch1 D3	GTGTCTTTATCTAGCTTCAATAG	1.69	3882-3904
6	Cch1 D3 nested	ATCCTCCTGGGCGCCATCGCACCG	1.69	3938-3961
7	Cch1 internal F	CGTCAGTCTATCCCGCTGCAAG	1.69	11036-11105
8	Cch1 internal R	GGTTAGGCAGAGGTGGTCTTGG	1.69	10166-10187
9	Cch1 internal F rc	CTTGCAGCGGGATAGACTGACG	1.69	11105-11036
10	HC-CN1004Fb	AACGCTGGTGAAAGTAAAAGATGCTGAA
11	HC-CN1004Rb	ACGCCTGGTTGCTACGCCTGAATAAGT
12	Hyg internal 3′	TTCGATCAGAAACTTCTCGACAGACG
13	Hyg internal 5′	CGGCCGTCTGGACCGATGGCTGTGTA

^aContigs and coordinates are given for the G. zeae genome and do not apply to hygromycin primers 10 to 13, which amplify pCB1004.

^bPrimers are courtesy of Jennifer Biezske.