FIG. 3.

Effect of sampangine on the growth of heterozygous mutants harboring deletions in heme biosynthetic genes. Wild-type (BY4743), HEM1/hem1Δ, HEM2/hem2Δ, HEM3/hem3Δ, HEM4/hem4Δ, HEM12/hem12Δ, HEM13/hem13Δ, HEM14/hem14Δ, and HEM15/hem15Δ strains were grown as described in Materials and Methods. Dilutions (fivefold) were prepared from each culture, inoculated onto SC agar medium, and incubated for 3 days at 30°C. −SMP, medium containing solvent (DMSO); +SMP, medium containing sampangine at 2.0 μg/ml.