FIG. 4.

Immunofluorescence localization of CrTOR, CrLST8, and BiP proteins in Chlamydomonas cells. (A) Exponentially growing wild-type cells were collected and processed for IF microscopy analysis as described in Materials and Methods. The antigens of interest are shown in green (FITC), whereas DNA staining is shown in blue (DAPI). Merge images show a merge of the green and blue channels. (B) Double staining of α-tubulin and CrTOR (top) or CrLST8 (down). CrTOR and CrLST8 are shown in green (FITC), α-tubulin in red (tubulin), and DNA in blue (DAPI). Merge images show a merge of the green, red, and blue channels.