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Lethocerus tropomyosin-TnT-TnH complex and TnC-F1 and F2. SDS-PAGE of samples: lane 1, IFM fraction applied to a butyl Sepharose column; lane 2, fraction from the column, containing pure TmTnTH; lane 3, F1 (TnC-F1); lane 4, F2 (TnC-F2); lane 5, markers. F1 and F2 were expressed in E. coli, and isolated from a cell lysate with Ni-agarose. An N-terminal 6His tag was removed with TEV protease. TnH is Lethocerus troponin I. αAc, α-actinin.
