TABLE 1.
Bacterial strains and plasmids used in this study
| Name | Genotype/relevant characteristics | Reference/source |
|---|---|---|
| E. coli strains | ||
| AG100 | argE3 thi-1 rpsL xyl supE44 λ lysogen | 15, also see reference 4; this studya |
| CV1008 | CV976 lrp-35::Tn10 (Tetr) | 32 |
| DT162 | MG1655 ΔgadX::kan | 43 |
| DT203 | MG1655 ΔgadW::kan | 43 |
| EF1155 | MG1655 ΔgadE(yhiE)::kan | 26 |
| GC4468 | Δ(lac)U169 rpsL | 9 |
| JHC1096 | GC4468 zdd-239::Tn9 del1738 (39 kb deleted, including marRAB locus) (Cmr) | 17 |
| LCB621 | MC4100 torR49::mini-Tn10 (Tetr) | 31 |
| PS2652 | ΔlacZ169 zch-506::Tn10 hns-1001::Tnseq1 (Kanr) | 6 |
| SPC105 | MC4100 marOII-lacZ (Ampr) in λatt site | 10 |
| ZK1000 | ZK126 ΔrpoS::kan | 7 |
| HdeA96 | JHC1096 hdeABp::lacZ (Ampr) in λatt site | This study |
| HdeA100 | HdeA96 transformed with pMB102 | This study |
| HdeA101 | HdeA100 hns-1001::Tnseq1 (Kanr) | P1 PS2652 × HdeA100 |
| HdeA103 | HdeA100 ΔrpoS::kan | P1 ZK1000 × HdeA100 |
| HdeA105 | HdeA100 lrp-35::Tn10 (Tetr) | P1 CV1008 × HdeA100 |
| HdeA107 | HdeA100 torR49::mini-Tn10 (Tetr) | P1 LCB621 × HdeA100 |
| HdeA109 | HdeA100 ΔgadX::kan | P1 DT162 × HdeA100 |
| HdeA111 | HdeA100 ΔgadW::kan | P1 DT203 × HdeA100 |
| HdeA113 | HdeA100 ΔgadE::kan | P1 EF1155 × HdeA100 |
| Plasmids | ||
| pHdeA415 | ori colE1 hdeABp-lacZ, AmpR | This study |
| pJPBH | ori colE1 lacI, AmpR | 37 |
| pMB102 | ori colE1 lacI lacZp::marA, AmpR | 33 |
| pRS415 | ori colE1 lacZ fusion vector, AmpR | 41 |
a
Determined in this study to be a single lysogen of λ by PCR as previously described (34).