FIG. 2.

iCF10 is insufficient to control endogenous pheromone in the absence of PrgY. Pheromone induction by endogenous pheromone was measured in OG1RF(pBK2) strains coharboring a plasmid expressing PrgZ+PrgY (pMSP6043), PrgY (pMSP6049), PrgZ (pMSP6043-1), or a vector control (pMSP6043-2). Cultures grown overnight in M9 medium were diluted 1:5, and β-galactosidase levels were determined after 2.5 h of growth. iCF10 was added at 0, 50, 500, or 5,000 ng/ml to overnight cultures and again to diluted cultures. The data shown are representative of three independent experiments, each performed in duplicate.