FIG. 5.

PrgY targets L4 of cCF10. Wild-type (WT) cCF10 peptide from pPCR4 or cCF10 variants were coexpressed with PrgY (pMSP3545Y) or a vector control (pMSP3545). The peptide produced by each strain during overnight growth in THB was collected in the supernatant and used to induce an exponentially growing responder strain OG1RF(p043lacZ) for 1.5 h, and the β-galactosidase activity was subsequently determined. Cell culture supernatants were added to the responder strain at a 1:100 dilution (for three cCF10 variants with low activity [V2L, L4V, and F6Y]) or at a 1:200 dilution (for all others). (A) Absolute values and (B) the PrgY-dependent percent reduction of cCF10 activity are shown. For both panels A and B, the error range represents the standard deviation of two independent experiments. MU, Miller units.