FIG. 7.

PrgY reduces the activity of cCF10 in the Eep-deficient strain JRC110. (A) cCF10 activity produced by pPCR4 expressed with pMSP3545Y (PrgY) or pMSP3545 (vector), as indicated, in strain JRC110 was measured by clumping assay as described in Table 3 and Materials and Methods. (B) PrgY-dependent percent reduction of cCF10 activity for JRC110 strains harboring pPCR4 (cCF10 processed from the iCF10 leader) or pJRC3 (cCF10 processed from the cAM373 leader). For both panels A and B, the results are averaged from three independent experiments done in duplicate (error bars represent the standard deviation of three independent experiments).