TABLE 1.
Primers used for PCR detection of genetic islands
| Locus | Primer sequencea
|
|
|---|---|---|
| Forward | Reverse | |
| hia | 5′-CCGAAAGCACAATGGATATGGACG-3′ | 5′-CAGATAAATCCTGACCTCGCTCTC-3′ |
| hmw1 | 5′-CAAAGCCATCAGGTTGTTGTGC-3′ | 5′-CCTATTTGGTCTTGCTACGAGTGG-3′ |
| hmw2 | 5′-CCGCACTTTCTTCTCGTTCTTCT-3′ | 5′-GCTATTCGGTTAGGTAATGCAGATCC-3′ |
| ahpC (tsaA) | 5′-CAGGGTTTACCGATCCTTGTGA-3′ | 5′-AGATAGCCATCTAGCCAGTCAGTG-3′ |
| HaeII | 5′-CATCCTTGGTTCTTATGGACAGCG-3′ | 5′-CTCTAATGGATCATCTCCGCTACG-3′ |
| R2846.1179-80 | 5′-CGGATCCACTCAATCTACTGCAAG-3′ | 5′-GAGTATCCCAACAAGATCTCAGCG-3′ |
| R2866.503-507 | 5′-CTGCGACGCATTTATTACAGGG-3′ | 5′-GTGGAATGCAAGGCTTAATGGG-3′ |
| R2866.1857-58 | 5′-CTGGAGCTTCATCTACCAATACGC-3′ | 5′-CAAGCAGAAACCCGTGAAATTATCG-3′ |
a
The primers shown for hia, hmw1, and hmw2 bind to sequences flanking the respective loci and were used to confirm the results of PCR using the internal primers described previously for these loci (11). The loci R2846.1179-80, R2866.503-507, and R2866.1857-58 encode putative restriction-modification systems of types II, I and III, respectively.