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. 2007 Dec 14;190(4):1284–1289. doi: 10.1128/JB.01599-07

FIG. 1.

FIG. 1.

Experiments with E. coli O157:H7 Sakai carrying an affinity-tagged RNAP β′ subunit. E. coli O157:H7 Sakai and a derivative carrying a chromosomal rpoC::PrA fusion were grown in MSM. (A) SDS-PAGE analysis of total cell extracts. Only the segment of the gel with the β′ subunit is shown. Lanes 1 and 3 show the tagged strain and lanes 2 and 4 show the wild-type strain. The proteins in lanes 1 and 2 were stained with Coomassie blue. Lanes 3 and 4 are from a Western blot probed with antibody specific for protein A. (B) Growth curve experiment comparing wild-type growth (dashed line) with the tagged strain (solid line). (C) SDS-PAGE analysis of proteins coisolated with RNAP:4PrA. Proteins were stained with Coomassie blue. Core RNAP subunits are indicated.