TABLE 1.
Analysis of CRISPR repeat sequences
| CRISPR | Type | Repeat sequence (5′-3′)a | No. of sequenced repeats | Frequency (%) |
|---|---|---|---|---|
| CRISPR1 | Typical repeat | GTTTTTGTACTCTCAAGATTTAAGTAACTGTACAAC | 2,820 | 99.7 |
| Repeat variants | GTTTTTGTACTCTCAAGATTTAAGTAACTGTGCAAC | 8 | 0.28 | |
| GTTTTTGTACTCTCAAGATTTAAGTAACCGTACAAC | 1 | 0.04 | ||
| Terminal repeat | GTTTTTGTACTCTCAAGATTTAAGTAACTGTACAGT | 107 | 87.0 | |
| GTTTTTGTACTCTCAAGATTTAAGTAGCTGTACAGT | 10 | 8.1 | ||
| GTTTTTGTATTCTCAAGATTTAAGTAACTGTACAGT | 6 | 4.9 | ||
| CRISPR2 | Typical repeat | GATATAAACCTAATTACCTCGAGAGGGGACGGAAAC | 129 | 74.6 |
| Repeat variant | GATATAAACCTAATTACCTCGAGAAGGGACGGAAAC | 44 | 25.4 | |
| Terminal repeat | GATATAAACCTAATTACCTCGAGAGGGGACTTTTTT | 59 | 100 | |
| CRISPR3 | Typical repeat | GTTTTAGAGCTGTGTTGTTTCGAATGGTTCCAAAAC | 670 | 99.8 |
| Repeat variant | TTTAACTCGCTGTGTTGTTTCGAATGGTTCCAAAAC | 1 | 0.15 | |
| Terminal repeat | Not different from the typical repeat |
a
Mutations as compared to the typical CRISPR repeat sequence are indicated by underscoring.