TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pUC4-KIXX | pUC derivative vector containing Kan cassette; Aprr | Pharmacia |
| pUC19 | Cloning vector; Ampr | |
| pKY75-1 | pUC19 1.8-kb BamHI fragment containing srrY | This study |
| pKY75-2 | pKY75-1 srrY::Kan | This study |
| pKAR4002 | pUC19 9.2-kb PstI fragment containing srrY | This study |
| pKAR3012 | pUC19 srrA | 1 |
| pKAU8202 | pUC19 srrA(W119A) | This study |
| pRES18 | E. coli-Streptomyces shuttle vector; Ampr Tsrr | 8 |
| pKY75-3 | pRES18 srrY::Kan | This study |
| pKAR3055 | pRES18 1.5-kb EcoRI-PstI fragment from pKAR3054 | This study |
| pKY71-1 | pRES18 2.2-kb SphI fragment containing srrZ | This study |
| pKY71-2 | pKY71-1 srrZ::Kan | This study |
| pTN03 | pRES18 srrW::Kan | This study |
| pKAU8203 | pRES18 srrA(W119A) | This study |
| Litmus28i | Cloning vector; Ampr | New England Biolabs |
| pKAR3023 | Litmus28i 1·5-kb AgeI fragment containing srrA from pKAR3012 | This study |
| pBluescript SK-plus | Cloning vector; Ampr | Stratagene |
| pKAR3001 | pBluescript SK-plus 1.7-kb Eco47III fragment containing srrW | This study |
| pRSET-B | Cloning vector; Ampr | Invitrogen |
| pKAR3053 | pRSET-B 1.8-kb EcoRI-PstI fragment from pKY75-1 | This study |
| pKAR3054 | pKAR3053 267-bp PvuII fragment deleted from srrY | This study |
| pTN01 | pRSET-B 1.7-kb EcoRI-BamHI fragment containing srrW from pKAR3001 | This study |
| pTN02 | pTN01 srrW::Kan | This study |
| pAlter-1 | Cloning vector for site-directed mutagenesis; Tetr | Promega |
| pKAR3025 | pAlter-1 KpnI and HindIII fragment of srrA from pKAR3023 | This study |
| pKAU8201 | pAlter-1 srrA(W119A) | This study |
| pET32b(+) | T7 expression vector for His10 tagging; Ampr | Novagen |
| pKAR3035 | pET32b(+) srrA | This study |
| pET-Amt | pET32b(+) srrA(W119A) | This study |
| pIJ8600 | Integrative E. coli-Streptomyces shuttle vector for PtipA expression; Aprr Tsrr | 28 |
| pKAR3049 | pIJ8600 srrY | This study |