FIG. 2.

Multiple alignment of some members of the SGNH family. ESPript (9) outputs were obtained from the BhRGAE, AaRGAE (Protein Data Bank accession no. 1deo), and hypothetical YxiM precursor (Protein Data Bank accession no. 2o14) sequences from the SWISSPROT databank (10) and aligned with CLUSTAL-W (32). Block I has the characteristic GDS sequence motif. Block II has a Gly as the only conserved residue in the members of this family. GXND is the consensus sequence in block III. Finally, block V has the DXXHP conserved sequence that is placed in a variable loop, whereas the other blocks are found at the C-terminal ends of the central β-sheet (20). Sequences are grouped according to similarity. Rectangles below the sequences show accessible residues in black, intermediate ones in gray, and buried ones in white. Residues strictly conserved among groups are shown in white font on a dark background. Residues conserved within a group but showing significant differences between groups are in gray font. The symbols above the blocks of sequences represent the secondary structure. Triangles represent the locations of the active sites.