FIG. 2.
Binding of the WT CheZC19 peptide to inactive CheY (A) or CheY activated with 0.1 M phosphoramidate (B). Peptide binding was assayed by measurement of intrinsic fluorescence of CheY Trp58 using excitation and emission wavelengths of 280 nm and 346 nm, respectively. The average scaled fluorescence intensities with standard errors of replicates are shown. The data for inactive CheY were generated with 10 μM CheY using slit widths of 0.6 nm and for P∼CheY, with 2 μM CheY using slit widths of 2 nm.