TABLE 2.
Construct | Primer | Sequence |
---|---|---|
CheAFL, CheAΔP2 | NdeI_forward | GGTGATCATATGAGCATGGATATTAGC |
CheAFL, CheAΔP2 | SalI_reverse | CTTCTCGTCGACTCAGGCGGCTGTGATCGC |
CheAΔP2 | BamHI_reverse | TGGGGATCCTGCTGCTGGTGCTGGCTGGATCGCCGCGCTTAG |
CheAΔP2 | BamHI_forward | GCAGGATCCCCACCACGAGCGTCAGCGGAACATCATGCGGGG |
CheAΔP2 | Linker_forward | TCGACGGATCCTCTAGA |
CheAΔP2 | Linker_reverse | AGCTTCTAGAGGATCCG |