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Binding analyses
| Peptide |
Kd (μM)a
|
|
|---|---|---|
| Inactive CheY | P∼CheY | |
| WTnonacCheZC19 | 206.9 ± 15.4 | 15.1 ± 0.1 |
| WT CheZC19 | 67.4 ± 4.5 | 2.2 ± 0.2 |
| CheZC19202Q→A,206D→A | 110.0 ± 9.2 | 19.5 ± 0.6 |
| CheZC19amide | 156.3 ± 12.5 | 7.9 ± 0.1 |
| CheZC19214F→A | 471.9 ± 26.0 | 53.2 ± 0.9 |
| WT CheZC15 | 64.7 ± 3.1 | 13.2 ± 2.1 |
Standard errors from triplicate experiments are listed.
