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Tandem SecA is functional and stable in vivo. (A) A complementation assay was carried out with BL21.19 cells harboring the vector pET5a alone, pET5a/secA, or pET5a/secAA (B) SDS-PAGE immunoblotting of SecA and SecAA from cells collected prior to and after SecA depletion (2 h after a shift from 30 to 42°C) with anti-SecA serum. One millimolar phenylmethylsulfonyl fluoride was added to cultures to prevent protein degradation.
