TABLE 3.
Relative abundances of various species from AUC sedimentation velocity
| Peak no. | Molecular mass (kDa)
|
Species | Relative abundance (%) at concn of HypE-HypF complex in AUC cell (mg/ml):
|
|||
|---|---|---|---|---|---|---|
| Theoretical | Estimated | 1.5 | 0.9 | 0.5 | ||
| 1 | 35.1 | 46 ± 5 | E | 5 | 9 | 9 |
| 2 | 81.9, 70.2 | 77 ± 6 | F or E2 | 11 | 9 | 20 |
| 3 | 117 | 110 ± 7 | EF | 72 | 70 | 52 |
| 4 | 163.8, 140.4 | 169 ± 8 | F2 or E4 | 6 | 8 | 15 |
| 5 | 234 | 215 ± 19 | [EF]2 | 6 | 5 | 3 |