TABLE 2.
Excision rates of IS256 from the spc gene, using a plasmid-based system
| Plasmid | Right/left TSD sequencea | IS256 sequence | Excision frequency (per cell generation)b |
|---|---|---|---|
| pTH1c | AGATCTAT/AGATCTAT | Full length | 1.5 × 10−11 |
| pTH2d | AGATCTAT/AGATCTAT | 166-bp fragment | 9.8 × 10−11 |
| pTH3c | AGATCTAT/AGATATTT | Full length | <2.0 × 10−13 |
| pTH4c | AGATCTAT/AGATTTGG | Full length | <4.7 × 10−13 |
| pTH5c | AGATCTAT/AGTTGGAA | Full length | <1.2 × 10−13 |
a
Mismatches between the TSDs are shown in bold.
b
Data are medians for all experiments.
c
Experiments were carried out in triplicate.
d
Experiments were repeated six times.