TABLE 3.
Primers used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| gtf2-5′ | CTTCTTGAGGAGCAATTCACAC |
| gtf2-3′ | AGGACAAGATCAATAACGCA |
| gtf2-PstIF | GATCCTGCAGATCCAAAGATTCTTGTGTGAGC |
| gtf2-PstIR | CTGCAGGTTAGATTTACTGGAACAATGTG |
| gtf-Spara-FF | GCGAGGATAAGTCGGAATCTG |
| Orf4D2-Spara-RR | AGGACAAGATCAATAACGC |
| gtf1-2-HindIII-IVS-RR | TCGAATAAGCTTCCGATCAGAACTCGC |
| gtf1-2-HindIII-IVS-FF | TGACAAGCTTACATTATCTGATTG |
| SalI-gtf1-FF | TGACGTCGACATGACAATCTATAATATTAATTTAGGG |
| BamHI-T7-gtf1-RR | TGACGCGGATCCCTAATCATTTAACATCTCC |
| SalI-gtf2-FF | TGACGTCGACATGATAGTTTTTGAATGGC |
| SalI-T7-gtf1-2-FF2 | GGACGTCGACATGGCTAGTATGACAGGAGGAGGTCAACAAATGGGAATGACAATC TATAATATTAATTTAGGG |
| KpnI-T7-gtf1-2-RR | TGACGCGGTACCATCTACATTACTAACCAATACCTGTTTATAATC |
| fap1-1814KpnI | GTTGAGGTACCGGTAGACTCTGATTTT |
| fap1-7080KpnI | GTTGAGGTACCGAAAGAACACTTCCG |
| BamHI-gtf1-FF | GATCGGATTCGAGGGAACAATGACAATC |
| XhoI-gtf2-RR | GATGCTCGAGGATTTTAACTACTAAATGGTC |
| gtf1-KpnI-IVS-RR | GACTGGTACCAGCATACTCAACACCGCTAC |
| gtf1-KpnI-IVS-FF | GATCGGTACCAAGCATGAAAAATTAGTTGAAAGATGG |
| gtf2-KpnI-IVS-RR | GACTGGTACCATCAAAGATTCTTGTGTGAGC |
| gtf2-KpnI-IVS-FF | GACTGGTACCTGGCGATCAGACTTATTTCCG |
| EcoRI-gtf1-FF | CGGAATTCATGACAATCTATAATATTAATTTAG |
| BamHI-gtf1-RR | CGGATCCATCATTTAACATCTCCTC |
| EcoRI-gtf2-FF | CGGAATTCATGATTAGGTTGTTTGAATGGC |
| BamHI-gtf2-RR | CGGATCCATCTACATTACTAACCAATAC |
| BglII-gtf2-FF | CAGGTTTAGATCTATCATC |
| pGEX6p1-gtf1-F(910) | AATCGGATCTGGAAGTTC |
| NotI/KAN-3 RP-2 | TCCCGTTGAATATGGCTCATAAC |
| gf2-T7-XhoI-RR | TGACCTCGAGTCCCATTTGTTGACCTCCTGTCATACTAGCCATATCTACATTACTAACCAA |
a
Restriction enzyme sites are underlined.