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. 2007 Dec 7;190(4):1366–1374. doi: 10.1128/JB.01621-07

FIG. 2.

FIG. 2.

Denaturing PAGE (12.5%) of various purification steps of heterologously expressed mesaconyl-CoA hydratase of C. aurantiacus and R. sphaeroides from extracts of 3 g E. coli cells. Lanes 1 and 7, molecular mass standards; lanes 2 to 4, Chloroflexus enzyme; lane 2, extract (100,000 × g supernatant) of induced E. coli cells (45 μg protein); lane 3, heat precipitation fraction (20 μg protein); lane 4, affinity chromatography fraction (8 μg protein); lanes 5 and 6, Rhodobacter enzyme; lane 5, extract (100,000 × g supernatant) of induced E. coli cells (30 μg protein); lane 6, affinity chromatography fraction (8 μg protein). The gel was stained with Coomassie brilliant blue R-250.