TABLE 6.
ORFs deduced from the genome of S. thermophilus phage 858 and their predicted functions
| ORF | Start | Stop | Size (aa)a | Mass (kDa) | pI | Putative ribosome-binding site and start codon (AAAGGAGGTGA) | Best matchesb (% amino acid identity) | Sizec (aa) | Putative function |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 319 | 525 | 68 | 8.1 | 10.5 | Not identified ATG | gp68 phage Sfi11 67/68 (98) | 68 | |
| 2 | 414 | 827 | 137 | 16.2 | 8.7 | CAGAGAGGTTAgtaca ATG | ORF1 phage 2972 137/137 (100) | 137 | |
| 3 | 1009 | 1461 | 150 | 16.8 | 6.7 | AAAATATTTGCggatgaatac TTG | ORF2 phage 2972 145/150 (96) | 150 | Terminase small subunit |
| 4 | 1448 | 2119 | 223 | 25.3 | 9.0 | AAAGGAGCTGTaagcg ATG | ORF3 phage 2972 223/223 (100) | 223 | Terminase large subunit |
| 5 | 2412 | 2999 | 195 | 22.7 | 4.8 | AGAGCCCTTGCgataacaaata ATG | ORF4 phage 2972 194/195 (99) | 195 | Terminase large subunit |
| 6 | 3008 | 4513 | 501 | 57.6 | 5.0 | GTAGGAGGAATg ATG | ORF5 phage 2972 501/501 (100) | 501 | Portal protein |
| 7 | 4510 | 5403 | 297 | 34.4 | 8.8 | TTGAGAGGGAAtatga ATG | ORF6 phage 2972 294/297 (98) | 297 | Capsid protein |
| 8 | 5591 | 6172 | 193 | 21.3 | 4.8 | TAGATAGGAGAaata ATG | ORF7 phage 2972 193/193 (100) | 193 | Scaffold protein |
| 9 | 6192 | 6551 | 119 | 12.8 | 8.8 | AAAGGAAATTTtaa ATG | ORF8 phage 2972 115/119 (96) | 119 | Capsid protein |
| 10 | 6570 | 7616 | 348 | 37.5 | 4.9 | AGAGGAGGAACattaaaac ATG | ORF9 phage 2972 346/348 (99) | 348 | Capsid protein |
| 11 | 7628 | 7789 | 53 | 6.0 | 9.3 | TTAAGAGGTACtgat ATG | ORF10 phage 2972 52/53 (98) | 53 | |
| 12 | 7801 | 8142 | 113 | 13.0 | 4.6 | TAGTGAGGTATggc ATG | ORF11 phage 2972 113/113 (100) | 113 | |
| 13 | 8139 | 8453 | 104 | 11.5 | 9.5 | AAAGAGGGAGAggtgttatttct ATG | ORF12 phage 2972 104/104 (100) | 104 | |
| 14 | 8455 | 8793 | 112 | 12.5 | 8.9 | CAAGGTGGTGAaataac ATG | ORF13 phage 2972 112/112 (100) | 112 | |
| 15 | 8795 | 9181 | 128 | 14.6 | 5.0 | AATGGCTAAGTgggaataag ATG | ORF14 phage 2972 127/128 (99) | 128 | |
| 16 | 9195 | 9704 | 169 | 18.5 | 4.8 | TCAGGAGGAAAaa ATG | ORF15 phage 2972 168/169 (99) | 168 | Tail protein |
| 17 | 9782 | 10135 | 117 | 13.2 | 4.7 | ATAGGAGTTAAaaaca ATG | gp117 phage Sfi11 101/117 (86) | 117 | |
| 18 | 10186 | 10503 | 105 | 12.6 | 9.9 | CGAGGAATTAAtcactaatgct ATG | ORF17 phage 2972 104/105 (99) | 105 | |
| 19 | 10493 | 15046 | 1517 | 153.4 | 9.6 | AGAGGGGCTTGctag ATG | ORF18 phage 2972 1511/1517(99) | 1517 | Tail protein |
| 20 | 15046 | 16581 | 511 | 57.7 | 5.3 | ATGAGAGGTATtaaata ATG | ORF19 phage 2972 508/511 (99) | 511 | |
| 21 | 16581 | 21434 | 1617 | 178.5 | 5.3 | ATTTGAGGAGAgatatatata ATG | ORF20 phage 2972 1599/1617(98) | 1605 | Receptor-binding protein |
| 22 | 21435 | 23456 | 673 | 74.2 | 5.7 | GTAGGAGGTTTttaa TTG | ORF21 phage 2972 659/673 (97) | 673 | Structural protein |
| 23 | 23473 | 23859 | 128 | 14.6 | 4.7 | AAGAAAGGAAAaatat ATG | ORF22 phage 2972 126/128 (98) | 128 | |
| 24 | 23885 | 24028 | 47 | 5.5 | 6.6 | TTAGGAGGAAGaaca ATG | ORF23 phage 2972 47/47 (100) | 47 | |
| 25 | 23991 | 24278 | 95 | 11.0 | 8.7 | CAAGAGCTTGTaggcttgtctc ATG | T. parva 22/87 (25) | 497 | |
| 26 | 24275 | 24409 | 44 | 5.0 | 10.7 | AAGCGAGGTTGataa ATG | S. pyogenes MGAS5005 19/35 (54) | 61 | |
| 27 | 24437 | 24691 | 84 | 9.3 | 9.5 | GAAACTTGAGAgg ATG | ORF87 phage Sfi21 68/79 (86) | 87 | Holin |
| 28 | 24693 | 25295 | 200 | 21.8 | 4.5 | TAAGGAAGGAAaatagt ATG | ORF25 phage DT1 180/200 (90) | 200 | Endolysin |
| 29 | 25341 | 25463 | 40 | 4.6 | 8.8 | AAACAAAGCGGtgtc ATG | ORF27 phage 2972 40/40 (100) | 40 | |
| 30 | 25502 | 26263 | 253 | 30.0 | 9.7 | AAAGATGGTGTcataag ATG | Phage J1 248/253 (98) | 253 | Endonuclease |
| 31 | 26325 | 26552 | 75 | 8.6 | 3.9 | AATCCCGGTTAca ATG | Phage J1 70/75 (93) | 186 | Endolysin |
| 32 | 26720 | 26851 | 43 | 5.3 | 8.9 | AAACGAGGTGAaaaca ATG | ORF30 phage 2972 43/43 (100) | 43 | |
| 33 | 26952 | 27161 | 69 | 7.9 | 7.9 | AAAGGAGAACTta ATG | ORF31 phage 2972 68/69 (98) | 69 | Cro-like repressor |
| 34 | 27178 | 27300 | 40 | 5.0 | 8.0 | AAAGGTATTTAaa ATG | ORF32 phage 2972 40/40 (100) | 40 | |
| 35 | 27557 | 28030 | 157 | 18.1 | 6.2 | TAGGGAGGGTAggaattaaat ATG | ORF33 phage 2972 157/157 (100) | 157 | |
| 36 | 28027 | 28728 | 233 | 26.2 | 6.6 | AAAGGAGAAACcttaacataag ATG | ORF34 phage 2972 233/233 (100) | 233 | |
| 37 | 28685 | 30097 | 470 | 54.2 | 8.6 | AAAGGGGTGTAaggtag ATG | ORF35 phage 2972 309/473 (65) | 445 | Helicase |
| 38 | 30104 | 30577 | 157 | 18.2 | 4.8 | AGATTTGGAGAtaaaaaaac ATG | S. suis 89/1591 110/157 (70) | 156 | |
| 39 | 30582 | 31397 | 271 | 30.7 | 7.8 | Not identified ATG | S. suis 89/1591 180/263 (68) | 268 | |
| 40 | 31366 | 32910 | 514 | 60.0 | 8.0 | AAAGGAGTTAGatactaaac ATG | S. suis 89/1591 363/508 (71) | 517 | Primase |
| 41 | 33167 | 33487 | 106 | 12.1 | 9.9 | AAAGAAAGGCAactttcaa GTG | ORF39 phage 2972 102/106 (96) | 106 | |
| 42 | 33698 | 33871 | 57 | 6.6 | 8.1 | GAAAGAGATGAtagaact ATG | ORF17 phage O1205 44/57 (77) | 57 | |
| 43 | 33868 | 34023 | 51 | 6.3 | 5.6 | GTAGGAGATTAgtagagtt ATG | ORF41 phage 2972 51/51 (100) | 51 | |
| 44 | 34024 | 34536 | 170 | 19.6 | 6.3 | TGAGGTGGAATag ATG | ORF42 phage 2972 170/170 (100) | 170 | DNA-binding protein |
| 45 | 34505 | 34831 | 108 | 12.1 | 9.2 | ATAGGAAAGGAaagatggtaa ATG | ORF43 phage 2972 108/108 (100) | 108 | |
| 46 | 34835 | 35542 | 235 | 27.7 | 9.1 | TGAGGAGTTATtaagac ATG | ORF44 phage 2972 234/235 (99) | 235 |
a
That is, the number of amino acids (aa) of the predicted protein.
b
If the host species is not mentioned, the phage infects a S. thermophilus strain.
c
That is, the size in amino acids (aa) of the best-matched protein.