Fig. 2. The effects of SP600125 on the period of Period1-luciferase bioluminescence rhythms in rat-1 fibroblasts.
Fibroblasts were synchronized by medium change and Period1-driven bioluminescence was recorded for several days. SP600125 (30 μM) lengthened the period of the bioluminescence rhythms. SP600125 negative control (30 μM) showed no significant effect. The left panels show the representative raw bioluminescence recordings and the right panels the baseline-corrected bioluminescence data. The columns in the lower panel show the estimated period length in each experimental condition. Values are means±S.E.M. *** P<0.005; ns, not significant. In all figures a number shown in a column indicates sample size.