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. Author manuscript; available in PMC: 2008 Mar 30.
Published in final edited form as: Neuroscience. 2007 Jan 30;145(3):812–823. doi: 10.1016/j.neuroscience.2006.12.037

Fig. 3. The effects of SP600125 on the acute and chronic activation of JNK in rat-1 fibroblasts.

Fig. 3

(A) Fibroblasts were synchronized by medium change with or without SP600125. The acute phosphorylation of p46 kDa and p54 kDa isoforms, measured 20 min after medium change was inhibited by SP600125. The lower panel shows quantitative data. Control (0 min) levels were normalized as 100. See detailed description in Fig. 1. (B) Cells were harvested on a full circadian cycle at 5-h intervals starting 30 h after medium change. p46 kDa and p54 kDa isoforms (open square), normalized toward the respective peak time level of expression in the control condition (shown in Fig. 1), did not show significant rhythmicity (both cases, P>0.01). Total amounts of both isoforms remained unvaried (both cases, P>0.1). Values are means±S.E.M. n=3 For each time point. Period1-luciferase rhythmic expression on the same time window is represented. * P<0.05; ** P<0.005; *** P<0.0005.