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. Author manuscript; available in PMC: 2008 Mar 30.
Published in final edited form as: Neuroscience. 2007 Jan 30;145(3):812–823. doi: 10.1016/j.neuroscience.2006.12.037

Fig. 4. Characteristics of SP600125 in the regulation of the Period1-driven bioluminescence rhythm in rat-1 fibroblasts.

Fig. 4

(A) Circadian rhythmic expression of Period1-bioluminescence in fibroblasts were synchronized and recorded for 3 days in the presence of 30 μM SP600125 (filled bar, 30.73±0.16 h). Medium was then replaced by assay medium without SP600125. After washout, the period was significantly shortened (24.44±0.10 h, P<0.0005). The graph is representative of three independent experiments. n=7. (B) SP600125 showed dose-dependent effects on the period lengthening. Data represent means±S.E.M. n=3–8 For each dose. Comparisons with the control condition (“0”): * P<0.05; *** P<0.005. Comparisons among SP600125 conditions: a P<0.05; c P<0.005. (C) SP600125 dose-dependently delayed the time of the onset. Data shown represent means±S.E.M. Comparisons with the control condition: * P<0.05; *** P<0.0005. Comparisons among SP600125 conditions: a P<0.05; b P<0.0005.