Figure 2.

CSFE-labeled, purified clone-4 TCR CD8⁺ T cells do not proliferate in the pancreatic lymph nodes after adoptive transfer into neonatal InsHA mice. Adult InsHA negative (A–C) and adult InsHA positive (D–F) mice were injected i.v., and 5-day-old neonatal InsHA mice (G and H) were injected i.p. with 5 × 10⁶ CSFE-labeled, purified clone-4 TCR CD8⁺ T cells. Seventy-two hours later cells isolated from various peripheral lymphoid organs as shown were stained with phycoerythrin-conjugated anti-CD8 antibodies. Data show amount of CSFE label among activated CSFE-labeled CD8⁺ T cells obtained from pooled lymphoid tissue taken from at least three adult mice per group and seven neonates. For clarity of depiction, the unlabeled cells have been deleted from the histograms.