Skip to main content
. 2008 Feb 15;22(4):436–448. doi: 10.1101/gad.1627008

Figure 2.

Figure 2.

Two distinct medulloblastoma cell populations survive 2 Gy of γIR. H&E staining for cells of the EN (A) and cells of the PVN (B) (red arrow) (SHH + N-myc representative pictures). (C) Cleaved caspase-3 and PCNA staining showing lack of cleaved caspase-3 and proliferation in the surviving cells of the PVN. Surviving PVN cells are adjacent to blood vessels, express nestin, and are cleaved caspase-3-negative (white arrows). (D) High- and low-magnification images of triple immunostaining with CD34 (red), nestin (green), cleaved caspase-3 (magenta), and DAPI counterstaining (blue) show that all surviving PVN cells are nestin-positive. Bars: A–C, 50 μm; D, 20 and 100 μm.