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. 2008 Feb 15;22(4):436–448. doi: 10.1101/gad.1627008

Figure 3.

Figure 3.

Evaluation of cell death and proliferation over time following 2 Gy of γIR in p53+/+ medulloblastomas. Cells surviving 2 Gy of γIR in the PVN re-enter the cell cycle at 72 h. (A) H&E, TUNEL, cleaved caspase-3, PCNA, and pH3 of untreated tumors and tumors treated by 2 Gy of γIR were collected after 4, 6, 12, 48, and 72 h, as indicated. Clusters of cells re-enter the cell cycle at 72 h (white arrow). (B) Triple immunofluorescent staining of PCNA (green), nestin (red), and DAPI (blue) at 72 h after 2 Gy of γIR in PVN shows high proliferation of nestin-positive cells (white arrows). Bars: A, 100 μm; B, 20 μm.