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. 2008 Feb 15;22(4):512–527. doi: 10.1101/gad.1631908

Figure 2.

Figure 2.

NHEJ efficiency and fidelity in wild-type and mutant M. smegmatis strains. Genotypes are indicated in the left column. NHEJ efficiencies for repair of transfected linear plasmids with blunt, 5′ overhang, and 3′ overhang DSBs in the M. smegmatis mutants were normalized to the wild-type values (100%). NHEJ fidelity is the fraction (percentage) of kanR transformants that were lacZ+. The fidelity values for wild-type M. smegmatis for repair of blunt, 5′ overhang, and 3′ overhang ends were calculated from counts of 4128, 2259, and 2626 kanR colonies, respectively. The fidelity values in the Δku mutant for blunt, 5′ overhang, and 3′ overhang NHEJ were calculated from counts of 143, 79, and 2169 kanR colonies. The fidelity values in the ΔligD strain for blunt, 5′ overhang, and 3′ overhang NHEJ were calculated from counts of 523, 1539, and 4953 kanR colonies. The fidelity values in the ΔligB/C/D strain for blunt, 5′ overhang, and 3′ overhang NHEJ were calculated from counts of 169, 126, and 1613 kanR colonies.