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. 2008 Feb 15;22(4):528–542. doi: 10.1101/gad.463208

Figure 1.

Figure 1.

Association between Trabid and APC proteins. (A,B) Immunoprecipitations between Flag-dTrbd and Drosophila APC (HA-E-APC) (A) or Flag-Trabid and an N-terminal truncation of human APC, GFP-APC(1–1447) (B), in cotransfected 293 cells, as indicated in panels. (C–E) Immunofluorescence of 293 cells cotransfected with HA-Trabid C443S and different GFP-tagged APC fragments, as indicated, fixed and stained with α-HA antibody (red in merge) and DAPI (blue in merge, to reveal the nuclei; see also Supplemental Fig. S5). Recruitment of GFP-APC(1–1447) into C443S puncta was observed in 9% of transfected cells (n = 100); neither APC(918–1698) nor APC(2068–2843) colocalized with these puncta (n = 100).