Figure 5.

Epistasis analysis of Trabid’s function in the Wnt pathway. TOPFLASH assays in 293T cells transfected with siRNAs as in Figure 4F and cotransfected with empty vector, HA-Wnt3A (A), stabilized Flag-β-catenin (Δ45S) (B), or 50–100 ng of catC-LEF1Δ56 or 1–5 ng VP16-LEF1ΔN chimeras (C), as indicated (the expression levels of the two chimeras were chosen to result in comparable transactivation). (Below) Western blots from a representative experiment to monitor expression levels of endogenous or overexpressed proteins (α-β-catenin antibody was used to detect catC-LEF1Δ56; VP16-LEF1ΔN was undetectable at these low expression levels); numbers below the blot in C indicate relative levels of catC-LEF1Δ56 expression (normalized to lane 2).