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. Author manuscript; available in PMC: 2008 Feb 11.
Published in final edited form as: Aging Cell. 2007 Nov 14;7(1):78–88. doi: 10.1111/j.1474-9726.2007.00355.x

Fig. 2.

Fig. 2

Sirt1 and uncoupling protein 2 (Ucp2) protein levels and stearoyl-CoA desaturase 1 (Scd1) and dipeptidyl peptidase IV (Dpp4) mRNA levels in islets isolated from aged BESTO and control mice. (A) Western blot analysis of Sirt1 and Ucp2 expression in transgenic (Tg) and control (Con) islet extracts from lines 431-2 and 431-4. Actin was used as a loading control for normalization. (B) Quantitation of the Ucp2 expression levels normalized to actin in BESTO transgenic islets (black bars) relative to control islets (white bars). (C) Quantitative real-time reverse transcriptase–polymerase chain reaction analysis of Scd1 and Dpp4 expression in BESTO transgenic islets (black bars) relative to control islets (white bars). The 3- to 5-month-old mice were considered young, while 18- to 19-month-old mice were considered old. All results are expressed as mean ± standard error. *P ≤ 0.05; **P ≤ 0.01.