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Redirected lysis of P815 targets by 2B4L or 2B4S transfectants. RNK-16 cells transfected with empty vector (RNK-16) or with 2B4 expression constructs (2B4L or 2B4S) were used as effectors in a standard chromium release assay against the murine FcR+ target P815. The effectors used are shown on the top of the figure. Effectors were preincubated with 25 μg/ml of isotype control antibody (cAb) or with anti-2B4 antibody before the addition of targets as indicated.
